Simultaneous extraction and enzymatic hydrolysis of mustard bran for the recovery of sinapic acid

The simultaneous extraction and enzymatic hydrolysis of sinapine from a mustard residue was designed for the recovery sinapic acid, high value-added phenolic acid. An initial screening allowed identification sinapoyl esterase activities in commercial cocktails (Depol 740 L, Ultraflo XL, Deltazym VR AC-100, Pectinase-PL “Amano”) mono-enzymatic solution rumen feruloyl esterase. These were not very tolerant to ethanol with diminution 70–90% activity presence 10% (v/v) ethanol. Different processes on bran depending solvent compositions (ethanol 70% (v/v), water or without esterase), pH (4.3, 7 12) temperatures (50, 75 100 °C). Their respective efficiencies discussed. implementation Depol L recover 68% accessible acid (25.4 ± 0.1 ?mol/g dry matter) 2h40 min under mild conditions (pH 7, 50 This efficient biocatalytic production feedstock using an cocktail paves way new developments design industrial process.